What Kind of Water is Suitable for Running HPLC-ECD Analysis
Purpose
This article outlines the requirements for what water can be used in HPLC-ECD analysis, including resistance, storage, and grading of the liquid.
Solution
Use ultrapure water (the resistance of the water needs to be at least 18.2 mega-ohm/cm). Any
solvent, such as mobile phase, or water applied to this system should be stored in a glass bottle. HPLC and LC-MS grade is best. Biological grades are not recommended as they are sometimes sterilized by UV – this can leave residual peroxides which can increase noise.
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