Washing Protocol for an AC-GEL Column
Purpose
This article outlines the protocol for washing an AC-GEL column and descriptions of the lifetime of the column.
Solution
AC-GEL Wash Protocol:
Remove the enzyme column, and re-pack the precolumn. Collect wash solution directly from the column-end. NEVER pass effluent through the detector cell.
Wash as follows:
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With distilled water for 60 min at the standard flow rate and in the forward direction.
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With 100% methanol for 60 min at the standard flow rate reverse direction.
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With distilled water for 60 min at the standard flow rate and in the forward direction.
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Run mobile phase for 60 min at the standard flow rate in the forward direction
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Connect to enzyme column and detector cell
Although it is possible to analyze more than a few thousand microdialysis samples between cleanings, it is only possible to analyze a few hundred samples of tissue homogenize. Thus, cleaning cycles will depend on what kind of samples are used.
. Please ensure that the enzymatic column is detached as the organic solvent used to clean the column can permanently damage the enzyme activity.
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