Tissue Homogenate Sample Preparation Protocol for Nitrate/Nitrite Analysis
Purpose
This article provides a guide to prepare homogenate samples for nitrite/nitrate analysis.
Solution
Add methanol at a concentration of 2 mL/g tissue.
Homogenize (in an ice bath, preferentially).
Centrifuge at 10,000 G for 10 min (at 4ºC, preferentially).
Inject the collected supernatant.
Note: If the concentration is too high, add the same volume of carrier solution to the supernatant.
Homogenize (in an ice bath, preferentially).
Centrifuge at 10,000 G for 10 min (at 4ºC, preferentially).
Inject the collected supernatant.
Note: If the concentration is too high, add the same volume of carrier solution to the supernatant.
The life span of the precolumn is about 100 injections of denatured protein samples taken from blood serum, plasma, or tissue.
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