How do I prepare the stretch chamber before seeding cells?

Please prepare the stretch chamber in this order:

1. Ultrasonic cleaning with surfactant 30-60 mins
2. Wash with purified water
3. Autoclave
4, Plasma treatment
5. Collagen coating
6. Incubation 3-5 hrs
7. Washing
8. Cell seeding
Derek is the author of this solution article.

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