Osmotic agents for preventing ultrafiltration during microdialysis

Dextran vs BSA:

Most of our users add 0.15% serum albumin as an osmotic agent as opposed to dextran, for several reasons:

-BSA is a better  blocking and carrier agent than dextran for preventing binding of peptides and proteins to plastic surfaces (probe, tubing, swivel, sample storage tubes).

-BSA acts as a sacrificial substrate for proteases in the perfusate.

-Dextran can cause a strong immune response if it leaks out of the probe. With 100 kDa MWCO probes, if you are going to use dextran, I would suggest using Dextran 500:


However, the presence of BSA makes LC-MS analysis much more difficult

Derek is the author of this solution article.

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