What is a normal pump pressure reading for each column?

Please note there are many variables that can change the pressure such as tubing length, temperature, and solvent composition.  Below is a guideline of the expected pressure range under normal conditions using mobile phase.

  • PP-ODS for DA and 5HT - 7.5 to 10 MPa
  • AC-GEL for ACh - 4.5 to 6 MPa
  • CA-5ODS for NE and E - 8.5 to 11 MPa
  • GU-GEL for Glutamate - 5 to 7 MPa
  • SC-5ODS for Monoamines - 6 to 9 MPa
  • SC-3ODS for Monoamines - 9 to 13 MPa.
  • AC-GEL for Acetylcholine- 4-6 MPa
  • CAX for NE, DA, 5-HT- 7 to 9 MPa
  • NOPAK (ENO-20) for Nitrite and Nitrate-1.2-1.8 MPa

Please also note that these values are just for reference.  

 

Higher pressure:

Possible causes

  • Time to repack the precolumn.
  • Time to wash/replace the separation column.
  • Clogging in the tubing/connection fittings.

 

Lower pressure 

Possible causes

  • Flow problem.  Check the measured flow rate compared to the set value.
  • Check all connecting tubing for leaks.
  • Air bubbles trapped in the pump (recommend purging).


D
Derek is the author of this solution article.

Did you find it helpful? Yes No

Send feedback
Sorry we couldn't be helpful. Help us improve this article with your feedback.