Split peak or broadened peaks, shorter retention times for PP-ODS columns

PP-ODS2 column needs to be washed. If the standard protocol as outlined in the user manual doesn’t solve the problem, proceed to wash the column as follows:

1. 30 min water in the reverse direction at half the normal flow rate.

2. 1 hr with 0.1% phosphoric acid in 100% acetonitrile in the reverse direction

3. 30 min water in the normal direction

4.  Run analytical mobile phase overnight to lower the BGC.

5. Re-check the separation.

Derek is the author of this solution article.

Did you find it helpful? Yes No

Send feedback
Sorry we couldn't be helpful. Help us improve this article with your feedback.