Split peak or broadened peaks, shorter retention times for PP-ODS columns

PP-ODS2 column needs to be washed. If the standard protocol as outlined in the user manual doesn’t solve the problem, proceed to wash the column as follows:

1. 30 min water in the reverse direction at half the normal flow rate.

2. 1 hr with 0.1% phosphoric acid in 100% acetonitrile in the reverse direction

3. 30 min water in the normal direction

4.  Run analytical mobile phase overnight to lower the BGC.

5. Re-check the separation.

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