Ringer’s Solution, aCSF Composition, Recipe
Purpose
The composition of Ringer’s solution and aCSF are detailed within this article, as well as precautions for when using the probes.
Solution
The following is a recipe for the perfusate of microdialysis Eicom recommends. Do not use any buffer solutions, as salts may precipitate and clog the probes.
Ringer’s Solution
8.6 g NaCl, 250 mg CaCl2, 300 mg KCl in 1 liter H2O
aCSF (artificial cerebro-spinal fluid)
147 mM (8.5 g) NaCl, 2.8 mM (208 mg) KCl, 1.2 mM CaCl2, 1.2 mM MgCl2
Can I use a buffer solution for microdialysis perfusate? No.
The salts in the buffered solution can precipitate out of the solution and clog the tubing. It is best to use the aCSF or Ringer’s solutions described above.
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