My Baseline Looks Like a Sine Wave
Purpose
This article covers multiple different solutions to removing the sine wave features from the baseline.
Solution
-
A sine wave on the chromatogram is typically caused by fluctuations in temperature.
-
Please ensure the HTEC door is closed securely.
-
If the system is placed close to an air conditioner, heater, or other drafts please move the system.
-
The HTEC’s temperature control sometimes shows small oscillations, even with the HTEC door latched. Please confirm the ECD has silicon tubing and a reference electrode cover installed as they are by default.
-
If those are installed correctly and the baseline oscillation persists, please raise the temperature up 1 degree.
-
If your reference electrode is over a year old, we recommend replacement.
-
If the reference electrode is relatively new but has the sine wave, please wrap the glass part of the electrode with a sponge for insulation. (Eicom provides the insulation material made with plastic for free, please contact us for this part.)