Is it Possible to Detect Acetylcholine in Brain Tissue Homogenate Using HPLC-ECD?
Purpose
This article concerns the feasibility of detecting Acetylcholine in homogenate and provides alternative procedures for both homogenate and microdialysis samples.
Solution
Acetylcholine is difficult to detect in brain tissue sample because of enzymes present, which quickly degrade acetylcholine even after sacrifice. While technically possible, the only known method to get accurate measurements in tissue sample is by microwave radiation in live animals. https://www.sciencedirect.com/
Thus, we cannot recommend acetylcholine detection in tissue samples. Instead, we recommend microdialysis for detection of acetylcholine.
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