I Observe a Large Ghost Peak before my GABA Peak
Purpose
This article outlines a resolution to a large ghost peak before a GABA peak.
Solution
At pH2.8 condition for GABA analysis, you will see a very large peak in front of the GABA peak. If the separation of those two peaks are not good, please raise the mobile phase pH at 0.2 increments. The retention time for the large peak slides towards the solvent front with a higher pH, whereas the retention time for GABA changes very little.
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