How do I Prepare the Stretch Chamber Before Seeding Cells?

Purpose

This article provides a procedure for preparing the stretch chambers for seeding.

Solution

Please prepare the stretch chamber in this order:

 

1. Ultrasonic cleaning with surfactant 30-60 mins
 

2. Wash with purified water

 

3. Autoclave

 

4, Plasma treatment

 

5. Collagen coating

 

6. Incubation 3-5 hrs

 

7. Washing

 

8. Cell seeding

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