How can Cell Proteins or mRNA be Obtained From the Strex Stretch Chamber after Culturing?
Purpose
This article outlines possible ways to remove proteins or mRNA after stretching including western blotting and immunoprecipitation.
Solution
1. Western blotting: Wash with PBS, add electrophoresis sample buffer directly to the stretch chamber, then collect the cell lysate with a cell scraper.
2. Immunoprecipitation: Wash with PBS, add cell solubilizer directly to the stretch chamber, then collect the cell lysate with a cell scraper.
3. mRNA: Wash with PBS suitable for RNA, add cell solubilizer directly to the stretch chamber, then collect the cell lysate with a cell scraper.
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