Cells Adhesion Appears to be Different From Chamber to Chamber. What Could be the Cause?
Purpose
This article provides a method to remove/prevent creases on the membrane of the stretch chamber.
Solution
In producing the stretch chambers, STREX works to assure that there are no creases or other imperfections on any of the surfaces. However, given the exceptionally thin membranes involved, creases can easily develop. Therefore it is important to use great care with the bottom surface when seeding the cells. To avoid creases, first treat a culture dish by dripping a bit of ethanol on it. Then set the chamber on the dish and tilt the dish and chamber, taking care not to introduce any air between the two. Allow some time for the ethanol to evaporate. The chamber will then be in optimal condition for seeding and culturing the cells with high adhesion.