This article provides a video and instructions on how to disassemble the pump head on an HTEC-510.
Tutorial-Disassembling HTEC Pump Head
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HTEC-ECD Articles
Tutorial – How to use the manual injector with the HTEC
A video tutorial and instructions on how to use and wash the manual injector are contained within this article.
What is the Particle Size for PP-ODS II or PP-ODSIII Columns?
The particle size of PP-ODSII and PP-ODSIII is 2μm.
What’s the Difference Between a Catecholamine Trap Column and a Precolumn?
This article explains how a catecholamine trap and precolumn filter different compounds/contaminants.
What Kind of Water is Suitable for Running HPLC-ECD Analysis
This article outlines the requirements for what water can be used in HPLC-ECD analysis, including resistance, storage, and grading of the liquid.
Is it Possible to Detect Acetylcholine in Brain Tissue Homogenate Using HPLC-ECD?
This article concerns the feasibility of detecting Acetylcholine in homogenate and provides alternative procedures for both homogenate and microdialysis samples.
Washing Protocol for an AC-GEL Column
This article outlines the protocol for washing an AC-GEL column and descriptions of the lifetime of the column.
How to Restart an HTEC System After a Long-Term Shutdown
The procedure outlined here would be applicable for an HTEC that has been out of use for over several weeks.
What is the Normal Pump Pressure Reading for Each Column?
This article outlines the nominal pressures for different columns as well as some common causes for a higher/lower observed pressure.
ECD Background, What is it, and What is the Standard Read?
This article explains what the ECD background current (BGC) is and how it affects sensitivity and analysis.
How Often Should I Replace the Reference Electrode in the HTEC?
This article covers the recommended length of time to replace a reference electrode.