Auto Zero: Confirming the Connection Between the AS-700 and the HTEC-500/510
Purpose
This article outlines the procedure to confirm an auto-zero signal is sent from the autosampler to the HTEC unit.
Solution
-
Please ensure the orange power strip is fully pushed in.
-
Check for loose cables.
-
Please touch the autozero terminals with forceps to short them on the orange strip of the HTEC-500. You should expect to hear a beep from the HTEC and you can see the baseline shifts to zero. If you cannot, the autozero circuit is broken. The value on the HTEC-500 display will not change with auto zero. Just the output value will change.
-
Then do the same thing on the Signal In of the HTEC-500. If you cannot hear the beep, there is a problem with the Signal In or wiring.
-
On the PC that is hooked to the HPLC, there should be a software called ASM (Alias Service Manager). Please close the chromatogram software and run ASM. ASM will communicate directly with the autosampler. Please select: ASM>Setting>Communication and choose the appropriate COM port. Then give a Output manually using ASM>Instrument>Maintenance Direct Control>Output and choose ON. You should be able to hear a clicking/switching sound from the autosampler. At the same time, you can hear a beep sound of autozero on the HTEC in 3 sec from the On.